Tuesday, October 17, 2017

Checking for Cleaning via Luminometer

Interesting data from University of Guelph research team on the connection between luminometer readings and laboratory bacteria culture results are found in this report:
"Validation of commercial luminometry swabs for total bacteria and coliform counts in colostrum-feeding equipment." (Journal of Dairy Science, 100:9459-9465 October 2017).

Using the test equipment available to the general public (Hygiena luminometer and various test swabs) they conclude that the Aqua-Snap Total and Microshap Coliform swabs are an acceptable alternative to traditional bacteria counts to evaluate cleanliness of colostrum-feeding equipment. About 59% of their surfaces has bacteria counts of at least 100,000cfu/ml - so they had plenty of dirty surfaces to check.

Note that they used rinse samples (15ml sterile water rinsed the surfaces to be tested).

At our vet clinic we use the Ultra-Snap testing swaps (direct collection from dry surfaces with moistened swabs). The data below are from a commercial dairy June 2016-July2017. Note the up and down pattern at certain sample sites. Acceptable ATP values were selected by the dairy.

Sanitation Audit Data
Acceptable ATP values: Feeding equipment <100, Environment<250
Location Sample Site Swab Site 14-Jun 14-Sep 13-Oct 10-Nov 22-Dec 30-Jun 26-Jul
2016 2016 2016 2016 2016 2017 2017
Milk room milker bucket newer inside 1/3 down 48 46 56 6 10 7 80
milker bucket older inside 1/3 down 259 45 17 11 10 11 121
plastic lid underside, under gask. 741 5298 118 93 34 41 58
Utility room Tube feeder inside bottle 2" - refrig 33 10 0 66 77 20 & 6 2&7
(calving) Tube feeder (ss) inside tube - top 2" 11 1404 0 4 59 3592 1
Tube feeder (ss) inside tube - ball 2" 995 3458 0 60 0 340 44
Holding pen plastic side - 1 ft.up 443 1167 555 1150 548 577 389
Garage Past. Milk discharge inside hose 2 inches 0 9 1 3 44 115 19
Gator Trans. Tank inside top 21 101 0 0 0 7 6
Trans. Tank end of hose 2 422 5 0 2 10 156
Trans. Tank feeding hose outlet 2 0 2 0 0 3 19&95
Nursing bottle inside bottle 2 inches 83 3027 n/a n/a 0 n/a 131
Nursing nipple inside surface 7 7976 n/a n/a 1363 n/a 208
Hutches Fdg. Pail 1 wk.(new) inside 1/3 down 144 2719 41 623 1 Rain 179
Fdg. Pail 1 wk.(old) inside 1/3 down 76 1919 24 500 0 Rain 33
Fdg. Pail 4 wks. inside 1/3 down 91 1020 213 225 90 Rain 1159*
Hutch surface 1 wk inside across from fdr 26 5989 925 289 209 Rain 621
Kane Grain fdr. 1 wk. front edge tray 41 240 74 30 191 Rain n/a
Kane Grain fdr. 4 wks. front edge tray 132 1158 428 54 59 Rain 1458
Bold = Needs Improvement *second pail = 2347

1 comment:

Cindy Kistler said...

Excellent post. I will certainly come back to read more of the articles about food. We love to try new receipts and new food, not only our native
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